​Gene gun method also known as particle bombardment, it seems to be slightly less popular, but is the only other one currently available to researchers. In this method, the created transgene cassette is placed in a vector (plasmid) and many copies of it are coated onto a microscopic gold-coated particle. This particle is accelerated into the plant cell nucleus, where the plasmids will detach from the particle and hopefully integrate into the plant genome. Transformation by particle bombardment is by no means permanent either, and like Agrobacterium transformation, also prone to unnecessary DNA such as vector DNA or the selectable marker transfer.

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